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KMID : 0377619740270050475
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Korean Jungang Medical Journal
1974 Volume.27 No. 5 p.475 ~ p.479
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An Experimental Study on Estimation of white Blood Cell Count in Bolldy Spinal Fluid
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Abstract
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Spinaial tapping is one of the common pediatric procedures and frequently used for the diagnosis of central nervous system diseases of children.
Bioody spinal taps are commonly encountered especially in the children who were very irritable and proper restraints were not performed.
Though blood contained specimens have little value for the calculation of white cells in the spinal fluid, white cells are frequently estimated by the proportion of white and red blood cells in peripheral blood of the children.
The object of this study is to evaluate the errors of such estimation of white cells in bloody spinal fluid. Spinal fluid was obtained from 25 cases of children who were admitted in pediatric ward, Severance Hospital and white and red cell counts were done with different mixture (concentration) of spinal fluid and pt´s own peripheral blood.
The results (calculation of white cells) were compared with original white cells in the spinal:´ fluid and t ha following results were obtained.
1) In general, estimation of white cell count with bloody spinal fluid on the basis of proportion of white and red cells in peripheral blood showed many errors and had only lirnitted values for such estimation.
2) If the more blood (RBC) introduced to the spinal fluid, the more errors were found in the estimation of white cells. The errors of white cell count in 1st mixture solution (less than 1, 000 RBC/mm3 in C.S.F) was 36% but errors of 2nd mixture solution (1, 0005, 000 RBC/mm3) and 3rd mixture solution (more than 5, 000 RBC/ mm´) showed errors of 56% and 80% respectively.
3) If the more white cells were present originally in C.S.F., the more accuracyof calculation was noted on white cell estimation after mixture of blood. Nearly no error or only 2 fold errors were noted in spinal fluid which was contained 100 or more cells per mm3 before mixture of blood. But 6 fold errors or more
were found in the spinal fluid which was contained only 0?10 white cells per mm´ in the origimal fluid.
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